Lipscomb Laboratory

Lipscomb Laboratory - Howard University

Research Detailed

Dendritic cell Biology and Antigen Presentation to T cells

Using transcriptomics and bioinformatics, the laboratory aims to identify and characterize novel genes expressed in dendritic cell (DC) subsets that govern both (1) differentiation from hematopoietic stem cells and (2) antigen presentation capacity to T cells. Using a combination of transcriptomic profiling, RNA interference, which include both siRNA- and CRISPR/Cas9-mediated silencing, and transgenic mouse models, mechanisms that govern the immunoregulatory pathway are delineated through biochemical, molecular, and cellular experimental approaches. The laboratory has uncovered novel roles of allograft inflammatory factor -1 (AIF1), A disintegrin and metalloprotease 23 (ADAM23), and drebrin 1 (DBN1) in mediating differentiation and/or antigen-presentation capacities of the cross-presenting subsets of CD11c+ dendritic cells. Continued studies aim to further uncover these proteins' functional role in the larger immnoregulatory pathways that dictate immunity vs. tolerance. Additional genes that show high correlative index in expression profile are also being pursued in the laboratory, which has now led into studies of macrophage differentiation and inflammatory roles in viral and parasitic infections. These studies are in part through collaborations with research teams at the Federal University of Segipe in Brazil.

Autoimmunity, Type 1 Diabetes, Immunotherapies and Insulin Restorative Therapies

Current therapies of type 1 diabetes are limited to non-specific global immune suppressive drugs, allogeneic transplantations, and administration of exogenous insulin. These approaches can cause a myriad of opportunistic infections and deregulation of host cellular processes (i.e. impaired endocrine signaling and disrupted hepatic functioning). The laboraotry's investigations aim to develop innovative immunotherapeutic approaches to inhibiting autoimmune destruction of beta-cells. The treatment application(s) employs sophisticated dendritic cell-based vaccine and adoptive T cell therapy strategies to induce antigen-specific tolerance to autoreactive T cells, as well as be used to suppress tissue-specific pro-inflammatory responses. In conjugation, methods of restorative insulin therapy using novel synthetic polymers for adoptive implantation therapies are also employed to restore physiologically-regulated/controlled insulin levels and alleviating hyperglycemia in diabetic individuals.

Michael W. Lipscomb

Michael Lipscomb is an Associate Professor in Department of Biology at Howard University. After attaining a BS from UCLA and PhD from Univ of Pittsburgh in Immunology, Dr. Lipscomb went on to setup a research laboratory at Howard University. There, his laboratory investigates basic immunoregulatory mechanisms associated with antigen presentation. Knowledge gained is then fed into studies developing immunotherapeutic approaches to combat type 1 diabetes.

Lipscomb Laboratory in Brief

Lab Members

Diana Elizondo, PhD - Research Scientist - ResearchGate Profile
Nailah Brandy, PhD - Research Associate - ResearchGate Profile
Ricardo da Silva, PhD - Visiting Professor - ResearchGate Profile 
Temesgen Andargie, MS - Graduate Student - ResearchGate Profile 
Leslie Goode, LATG - Animal Technician 

Extramural Awards

DEVELOPMENT OF TOLEROGENIC DENDRITIC CELL-BASED IMMUNOTHERAPIES AND RESTORATIVE INSULIN APPROACHES TO ALLEVIATE TYPE 1 DIABETES

Award Number: SC1GM127207
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH

CROSSTALK AND THE CYTOSKELETON IN DENDRITIC CELL ANTIGEN PRESENTATION

Award Number: SC2GM103741
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
 

MRI: Acquisition of Flow Cytometric Analyzer for Research and Training at Howard University

Award Number: 1428768
ORGANIZATION: NATIONAL SCIENCE FOUNDATION

Recent Publications

Elizondo DM, Brandy NZD, da Silva RLL, Haddock NL, Kacsinta AD, de Moura TR, Lipscomb MWAllograft Inflammatory Factor-1 Governs Hematopoietic Stem Cell Differentiation Into cDC1 and Monocyte-Derived Dendritic Cells Through IRF8 and RelB in vitro. Front Immunol. 2019 Feb 8;10:173. doi: 10.3389/fimmu.2019.00173. eCollection 2019.

Elizondo DM, Andargie TE, Haddock NL, da Silva RLL, de Moura TR, Lipscomb MWIL-10 producing CD8+ CD122+ PD-1+ regulatory T cells are expanded by dendritic cells silenced for Allograft Inflammatory Factor-1. J Leukoc Biol. 2019 Jan;105(1):123-130. doi: 10.1002/JLB.1A0118-010RR. Epub 2018 Dec 4.

Elizondo DM, Andargie TE, Haddock NL, Boddie TA, Lipscomb MWDrebrin 1 in dendritic cells regulates phagocytosis and cell surface receptor expression through recycling for efficient antigen presentation. Immunology. 2019 Feb;156(2):136-146. doi: 10.1111/imm.13010. Epub 2018 Nov 8.

Magalhães LS, Bomfim LG, Mota SG, Cruz GS, Corrêa CB, Tanajura DM, Lipscomb MW, Borges VM, Jesus AR, Almeida RP, Moura TR. Increased thiol levels in antimony-resistant Leishmania infantum isolated from treatment-refractory visceral leishmaniasis in Brazil. Mem Inst Oswaldo Cruz. 2018 Feb;113(2):119-125. doi: 10.1590/0074-02760170289.

Elizondo DM, Andargie TE, Yang D, Kacsinta AD, Lipscomb MW. Inhibition of Allograft Inflammatory Factor-1 in Dendritic Cells Restrains CD4+ T Cell Effector Responses and Induces CD25+Foxp3+ T Regulatory Subsets. Front Immunol. 2017 Nov 8;8:1502. doi: 10.3389/fimmu.2017.01502. eCollection 2017.

Elizondo DM, Andargie TE, Kubar DM, Gugssa A, Lipscomb MWCD40-CD40L crosstalk drives fascin expression in dendritic cells for efficient antigen presentation to CD4+ T cells. International Immunology. 2017

Elizondo DM, Andargie TE, Kubhar DS, Marshall KM, Zariwala AM, Lee CM, Lipscomb MWsiRNA Immunological Fishing Training (SIFT) Experience as a Novel Research Education Tool for Students Studying Immunology. J Microbiol Biol Educ. 2017 Apr 21;18(1). pii: 18.1.110. doi: 10.1128/jmbe.v18i1.1288. eCollection 2017 Apr.

Silva RL, Santos MB, Almeida PL, Barros TS, Magalhães L, Cazzaniga RA, Souza PR, Luz NF, França-Costa J, Borges VM, Lima-Junior DS, Lipscomb MW, Duthie MS, Reed SG, Almeida RP, Jesus AR. sCD163 levels as a biomarker of disease severity in leprosy and visceral leishmaniasis. PLoS Negl Trop Dis. 2017 Mar 29;11(3):e0005486. doi: 10.1371/journal.pntd.0005486. eCollection 2017 Mar.

Lays Gisele Santos Bomfim, Lucas Sousa Magalhães, Marcelo Augusto Anchieta Santos Filho, Nalu Teixeira de Aguiar Peres, Cristiane Bani Corrêa, Diego Moura Tanajura, Angela Maria Silva, Michael Wheeler Lipscomb, Valeria Matos Borges, Amélia Ribeiro Jesus, Roque Pacheco De Almeida, Tatiana Rodrigues De Moura. Leishmania infantum induces the release of sTREM-1 in visceral leishmaniasis. Frontiers in Microbiology. 2017

Roseane Nunes de Santana Campos; Márcio Bezerra Santos; Gabriel Isaias Lee Tunon; Luana Celina Seraphim Cunha; Lucas Sousa Magalhães; Michael Lipscomb; Juliana Lima Silva Moraes; Danielle Melo S. Ramalho; Sanmy Silveira Lima; José Antônio Pacheco de Almeida; Amélia Ribeiro de Jesus; Roque Pacheco de Almeida. Epidemiological aspects and spatial analysis of human and canine visceral leishmaniasis in an endemic area in north-eastern Brazil. Geospatial Health. 2017

Elizondo DM, Andargie TE, Marshall KM, Zariwala AM, Lipscomb MW.  Dendritic cell expression of ADAM23 governs T cell proliferation and cytokine production through the α(v)β(3) integrin receptor. J Leukoc Biol. 2016 Nov;100(5):855-864. Epub 2016 Jun 17.

Mwendwa DT, Ali MK, Sims RC, Cole AP, Lipscomb MW, Levy SA, Callender CO, Campbell AL. Dispositional depression and hostility are associated with inflammatory markers of cardiovascular disease in African Americans. Brain Behav Immun. 2013 Feb;28:72-82

Equipment and Facilities

- 1 BD FACSVerse flow cytometric analyzer (405, 488, and 640nm laser excitation; 10-parameter) 
- 1 BD FACSJazz flow cytometric sorter (488 and 640nm laser excitation; 7-parameter)
- 1 BD Accuri C6 flow cytometric analyzer (488 and 640nm laser excitation; 6-parameter) 
- 1 Luminex Magpix multiplex microplate reader system with xMAP technology 
- 1 Biotek HT multimode fluorescence, luminescence, and absorbance microplate reader 
- 1 Licor Odyssey infra-red two laser-scanning nucleic acid and protein imaging system 
- 1 Cryostar NX70 cryogenic sectioner with full automation 
- 1 Olympus FSX100 (3-Channel; 350, 488, 561nm) XYZ-motorized fluorescent microscope 
- 1 Olympus SZ61 40X Stereomicroscope with attached Digital Camera (488 and 561nm) 
- 2 EVOS FL manual inverted microscopes (DAPI, GFP, Cy5.5, Cy7 Texas Red) 
- 1 Countess II automated fluorescent cell counter (DAPI, GFP, RFP, Texas Red, Cy5.5, and Cy7) 
- 1 Thermo Fisher QuantStudio 5 Real Time qPCR machine
- 1 Thermo Fisher Veriti Gradient thermocycler
- 1 BioRad T100 thermocycler
- 1 NanoDrop Lite connected to 1 laptop
- 1 ECM 830 square wave electroporation unit
- 1 Miltenyi GentleMACS Dissasociator 
- 1 Thermo Fisher Scientific 5 ft. Class II Type A2 Biological Safety Cabinet
- 2 Thermo Fisher Scientific CO2 incubators; 1 direct heat injection and 1 water-jacketed
- 1 Quincy 140 series standard incubator dedicated for bacterial cultures
- 1 Allegra X-12R refrigerated benchtop centrifuge with microplate/tube interchange assembly
- 1 Allegra X-30 benchtop centrifuge with microplate/tube interchange assembly
- 2 Microcentrifuge-16 centrifuges; 1 refrigerated and 1 room temperature
- 1 MaxQ Orbital shaker incubation unit; fits 250 mL and 50 mL flask sizes
- 1 UV/Visi Blue Protein and Gel Blot Reader
- 1 Benchmark BioClave™ Research Benchtop Autoclave
- 6 power supplies for (nucleic acid and protein) electrophoresis apparatuses
- 1 Thermo Scientific Locator Jr Liquid Nitrogen Dewar
- 1 minus 86 C ultralow freezer with digital ambient recorder
- 1 minus 20 C freezer with digital ambient recorder
- 1 4C dual refrigerator with digital ambient recorder
- 1 4C under counter refrigerator dedicated to antibodies and bioreagents 

Recent News and Events in the Lipscomb Laboratory

Ricardo da Silva

Ricardo da Silva
January 15, 2019

Welcome Ricardo da Silva to the Lipscomb Laboratory

Ricardo L. da Silva is a professor from Universidade Federal de Sergipe. He recently received his PhD in the laboratory of Roque Almeida and Amelia de Jesus, who are collaborators with Michael Lipscomb. After Ricardo and Dr. Lipscomb worked closely in Brazil, Ricardo came to pend a year studying at Howard University in the Lipscomb Laboratory. His projects is delineating the role of AIF1 in differentiation of macrophages from hematopoietic precursors and larger role in controlling innate immune responses in vvio

Diana Elizondo, PhD

Diana Thesis
May 15, 2018

Successful PhD Thesis Defense by Diana Elizondo

Congratulations to Dr. Diana Elizondo, who has successfully graduated with a PhD from the Lipscomb laboratoy and is now a postdoctoral fellow in the laboratory of Carey Lumeng at Univ. of Michigan! In continuing her studies of the interface of immunity and diabetes, Dr. Elizondo has now transitioned to studying the role of macrophages in contribution to type 2 diabetes and obesity. 

While at Univ of Michgian, Diana has won a slot in the T32 training grant Multidisciplinary Postdoctoral Training Program in Basic Diabetes Research (T32DK101357) with the 2019-2020 cohort

Nailah Z. D. Brandy

FACSJAzz
Nov 15, 2018

Welcome Nailah Brandy to the Laboratory

Dr. Nailah Brandy is a new research associate in the Lipscomb laboratory, having earned her PhD in chemistry and completing her first round of postdoctoral studies. She has transitioned from organic chem synthesis to studying antigen-presenting myeloid cell contribution to immunity and differentiation from HSC.

Pancreatic beta cells seeded in bioscaffold form organoid in vivo to rescue type 1 diabetes

EurekaAlert!

The Endocrine Society - Published on Mar 19, 2018 - This press conference explores efforts to reverse diabetes with implanted beta cells, the development of a wearable patch to detect dangerous episodes of low blood sugar called hypoglycemia, and the effectiveness of a hybrid closed-loop system for managing type 1 diabetes

See below the video

Lipscomb Laboratory Team - April 2019

Lipscomb Laboratory Team - 2019

Michael Lipscomb, PhD, Diana Elizondo, PhD, Ricardo Louzada da Silva, PhD, Nailah Brandy, PhD, and Leslie Goode. not pictured: Temesgen Andargie, MS,

Lipscomb Laboratory Environment

Lipscomb Laboratory Embraces Diversity

GraduateStudents1

#DayWithoutImmigrants

Lipscomb Laboratory's Diana Elizondo, PhD, graduated in May 2018 and current graduate student Temesgen Andargie, MS, engage in scientific discussions. Culture and heritage intertwined with impactful science is the motto